Root - SRA - NCBI

SRX8587415: Root
1 ILLUMINA (Illumina HiSeq 2000) run: 28.9M spots, 8.7G bases, 2.5Gb downloads

Design: Total RNA was extracted from collected tissues using the RNAprep pure plant kit (TIANGEN, China). Qualified RNA per sample was used to generate sequencing libraries by NEBNext Ultr RNA Library Prep Kit for Illumina (NEB, USA) following manufacturer's instruction. mRNA was purified from total RNA using poly-T oligo-attached magnetic beads, then interrupted into short fragments which were used as templates for cDNA synthesis. After purification, reparation, adenylation and adapter ligation of 3'end, 150200 bp of cDNA fragments were separated for PCR amplification. At last, the libraries were quality control by Agilent 2100 Bioanalyzer and Q-PCR.

Submitted by: Shanghai Chenshan Botanical Garden

Study: RNA-Seq of different tissues from Tripterygium wilfordii

PRJNA640746 • SRP268120 • All experiments • All runs

show Abstracthide Abstract

Illumina HiSeq and Pacbio squencing of different tissues from Tripterygium wilfordii, using for genome annotation.

Sample:

SAMN15331501 • SRS6878884 • All experiments • All runs

Organism: Tripterygium wilfordii

Library:

Name: R2

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq